EUCOMM Training Event - Genetic Manipulation of ES Cells
26 October – 8 November 2009
This course provided a comprehensive overview and practical laboratory experience of the genetic manipulation of ES cells for a broad range of applications. Attendees were in the main postdoctoral scientists and senior P hD students actively engaged in research. The course covered a range of topics including recent advances in genome informatics, BAC recombineering, transposon technology and conditional gene targeting. Practical elements included:
- recombineering of conditional gene targeting vectors
- ES cell culture
- gene trap mutagenesis
- conditional gene targeting
- informatics
Course Instructors
- Professor Allan Bradley (Wellcome Trust Sanger Institute, Cambridge)
- Professor Francis Stewart (Dresden University of Technology, Dresden)
- Dr William Skarnes (Wellcome Trust Sanger institute, Cambridge)
- Dr Pentao Liu (Wellcome Trust Sanger Institute, Cambridge)
- Dr Barry Rosen (Wellcome Trust Sanger Institute, Cambridge)
Guest Speakers
Andras Nagy (Samuel Lunenfeld Research Institute, Toronto, Canada), Andrew Smith (University of Edinburgh, UK), Austin Smith (Wellcome Trust Centre for Stem Cell Research, Cambridge, UK), Drew Murphy (Regeneron, NY, USA), Eugene Yu (Roswell Park Cancer Institute, NY, USA), Junji Takeda (Osaka University, Japan).
Further Details
Please note the following files are very large.
Group presentations (zip file).
Instructors' presentations (zip file).