EUCOMM Practical Informatics Training Day
1st December 2008
UPDATE: The presentation slides from the training day are attached to the bottom of this page.
A world wide mutagenesis effort is underway aimed at mutating virtually all mouse genes for functional annotation of the genome. The cornerstones of this international effort are the European EUCOMM project, the U.S. KOMP project, the Canadian NorCOMM project and the Texas Institute for Genomic Medicine (TIGM), which collectively form the International Knockout Mouse Consortium (IKMC). The IKMC projects are exchanging data and materials to establish a unique, comprehensive resource useful for scientists all over the world.
To this international initiative, EUCOMM will contribute the largest fraction of conditionally mutated genes and was the first project to recognize the importance of conditional mutagenesis.
The international community is invited to make the most of the EUCOMM resource by participating in a one-day EUCOMM training course. This course aims to enable researchers to use and understand the extensive bioinformatics infrastructure underpinning the EUCOMM pipeline. The course will be held at the Wellcome Trust Sanger Institute.
Aimed at non-EUCOMM participants the course will cover the following topics:
-
A review of the EUCOMM production pipeline for making vectors for targeted conditional knockouts:
- Targeting ES cells
- The structure of conditional knockouts, deletions and insertions
- Recombineering using the KO design
- Gateway and beyond
- Using the EUCOMM design tool to make your own recombineering designs
- QC of targeting vectors and ES cells
- Mapping and annotating EUCOMM gene-trap tags
- Retrieving information from the EUCOMM website on genes, constructs and ES cells
The course will take place on December 1st, 2008 in the Rosalind Franklin Pavilion at the Hinxton Hall Conference Centre, nr Cambridge, UK. Places and accommodation are available for up to 40 people on a first-come-first-served basis.
A registration fee of €223.00 is payable, which covers accommodation and training; a limited number of bursaries will be available for students and postdoctoral researchers.
To book your place, please contact eucomm@sanger.ac.uk.
Further information on travel and the venue can be found at http://hydra.hinxton.wellcome.ac.uk/introduction.html.
Presentation Slides
- Introduction - Bill Skarnes
- Mutant ES Cell Data: Accessing It With Your Computer - Vivek Iyer
- Gene Annotation and KO Design - Alejandro Mujica
- Understanding Modular EUCOMM Gene Targeting Vectors and Their Functional Annotation - Barry Rosen
- ES Cell Clone QC - Tony West
- Random Gene Traps: Interpreting Sequence Tags - Vivek Iyer