EUCOMM Training Event - Manipulation of Mouse ES Cells
6 July 2007
Franz Volhard Auditorium (22-2), University of Frankfurt Medical School,
Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.
Description of Course
As part of the world-wide efforts to acquire a comprehensive understanding of a mammal, the EUCOMM program is adapting mouse ES cell technologies for high throughput, systematic, mutagenesis. This course provides the theoretical background of the tools and strategies encompassed by the program, in a review format.
EUCOMM members as well as outside participants are welcome. The course is scheduled for ONE FULL DAY from 9AM - 5.30 PM.
For further questions please contact Ms. Doris-Maria Viel at D.M.Viel@em.uni-frankfurt.de
Programme
Timetable| 09.00 - 09.10 | Welcome Harald von Melchner (Frankfurt, Germany) |
|---|---|
| 09.10 - 09.40 | Derivation of ES cells from mice Jenny Nichols (Cambridge, UK) |
| 09.40 - 10.10 | ES cell culture and handling William C. Skarnes (Hinxton, UK) |
| 10.10 - 10.40 | Gene trapping in ES cells Harald von Melchner (Frankfurt, Germany) |
| 10.40 - 11.10 | Coffee break |
| 11.10 - 11.40 | Characterization of gene trap insertion sites Patricia Ruiz (Berlin, Germany) |
| 11.40 - 12.10 | Gene targeting in ES cells Roland Friedel (Neuherberg, Germany) |
| 12.10 - 12.40 | Site specific recombination Francis Stewart (Dresden, Germany) |
| 12.40 - 13.10 | Recombineering and combinatorial engineering Francis Stewart (Dresden, Germany) |
| 13.10 - 14.00 | Lunch |
| 14.00 - 14.30 | Conditional gene targeting Barry Rosen (Hinxton, UK) |
| 14.30 - 15.00 | Conditional gene trapping Frank Schnütgen (Frankfurt, Germany) |
| 15.00 - 15.30 | Recombinase mediated cassette exchange in ES cells Ralf Kühn (Neuherberg, Germany) |
| 15.30 - 16.00 | Inducible systems for conditional mutagenesis Rolf Sprengel (Heidelberg, Germany) MPEG-4 Video File |
| 16.00 - 16.30 | Coffee break |
| 16.30 - 17.00 | Derivation of mice from mutant ES cells William C. Skarnes (Hinxton, UK) |
| 17.00 - 17.30 | Conditional RNAi gene knock downs in mice Jost Seibler (Köln, Germany) |
| 17.30 | Wine and cheese |
Outcome
The course had 65 participants and was a great success. The talks from the course are added above. Click on a talk title above to open the pdf version of the presentation.
