Protocols Used by the EUCOMM Project

• Gateway Protocols
  • High Throughput Gateway (96 Well) Reactions
  • Three Way Gateway Reaction (in Tubes)
• Tissue Culture Protocols
  • Feeders for F6 Cells
  • Protocol for Colony Picking
  • Preparing DNA for Electroporation
  • General Instructions for Single Cuvette Electroporation With DNA Targeting Vectors
  • N4 Protocols
    • Medium and Reagents for JM8.N4 (C57/Bl6/N) ES Cells & Feeders
    • Expanding JM8.N4 for Electroporation
    • JN8.N4 Cells Electroporation Protocol
    • Passaging/Splitting Cells
  • F6 Protocols
    • Medium and Reagents for JM8.F6 (C57/Bl6/N) ES Cells & Feeders
    • Expanding JM8.F6 for Electroporation
    • JM8.F6 Cells Electroporation Protocol
    • Passaging/Splitting Cells
• Geneotyping Protocols
  • ES Cell Genomic DNA Purification in 96 Well Format
  • Exonuclease I/Phosphatase
  • 384/96 Well Long Range Polymerase Chain Reaction (LRPCR)
  • Sequencing Reaction Setup
  • Genotyping protocol (Expand Long Template)
• Mouse Production Protocols
  • Chimera Mouse Production by Blastocyst Injection