Protocols Used by the EUCOMM Project

• Genomic DNA purification in 96 well
• Three Way GW Reaction
• Preparing DNA for electroporation
• Medium and Reagents for JM8.F6 ES cells
• ES cell protocols
• Expanding JM8 F6 cells for electroporation
• Electroporation of JM8.F6 cells
• Passaging electroporated JM8.F6 ES Cells
• Genotyping protocol