Project Information
The EUCOMM Integrated Project is funded by the European Union Framework 6 programme. Together with two other major mouse knockout programs (KOMP funded by NIH and NorCOMM funded by Genome Canada), EUCOMM is participating a worldwide effort to generate mutations in all protein-encoding genes in the mouse. This combined effort will be achieved by using a combination of gene trapping and gene targeting in mouse embryonic stem (ES) cells. The goal of EUCOMM is to generate a collection of up to 20,000 mutated genes in C57BL/6 N mouse ES cells using conditional gene trapping and gene targeting approaches. This represents a significant contribution towards the establishment of a global mouse mutant library in a standardized and cost-effective manner, making mouse mutants available to a much wider biomedical research community than has been possible previously.
The scientific and technological objectives of EUCOMM are the generation and distribution of:
- up to 12,000 conditional gene trap mutations in ES cells
- up to 8,000 targeted conditional mutations in ES cells in genes which cannot be readily trapped by random gene trapping methods
- Inclusion, in the conditional targeting approach, of human disease genes
- Establishment and archiving of up to 320 mutant mouse lines for quality assurance and for EU-funded phenotyping programmes
- Establishment of up to 20 ligand-inducible Cre-recombinase expressing transgenic mouse lines which differ in their expression characteristics
- Establishment of an online database to disseminate EUCOMM material worldwide
- Integration of the EUCOMM database with other bioinformatics resources
- Training of technical staff, students and postdocs
- Generation of a European mouse mutagenesis platform and forum, synergistic integration of EMMA, EUMODIC, EURExpress, FLPFLEX, EMAGE, FunGenES, PRIME and others
Technology
Publications