EUCOMM Training Event

Manipulation of mouse ES cells

6 July 2007

Franz Volhard Auditorium (22-2), University of Frankfurt Medical School, Theodor-Stern-Kai 7, 60590 Frankfurt/Main, Germany.

Description of Course

As part of the world-wide efforts to acquire a comprehensive understanding of a mammal, the EUCOMM program is adapting mouse ES cell technologies for high throughput, systematic, mutagenesis. This course provides the theoretical background of the tools and strategies encompassed by the program, in a review format.

EUCOMM members as well as outside participants are welcome. The course is scheduled for ONE FULL DAY from 9AM - 5.30 PM.

For further questions please contact Ms. Doris-Maria Viel at D.M.Viel@em.uni-frankfurt.de

Programme

Timetable

09.00 - 09.10	Welcome Harald von Melchner (Frankfurt, Germany)
09.10 - 09.40	Derivation of ES cells from mice Jenny Nichols (Cambridge, UK)
09.40 - 10.10	ES cell culture and handling William C. Skarnes (Hinxton, UK)
10.10 - 10.40	Gene trapping in ES cells Harald von Melchner (Frankfurt, Germany)
10.40 - 11.10	Coffee break
11.10 - 11.40	Characterization of gene trap insertion sites Patricia Ruiz (Berlin, Germany)
11.40 - 12.10	Gene targeting in ES cells Roland Friedel (Neuherberg, Germany)
12.10 - 12.40	Site specific recombination Francis Stewart (Dresden, Germany)
12.40 - 13.10	Recombineering and combinatorial engineering Francis Stewart (Dresden, Germany)
13.10 - 14.00	Lunch
14.00 - 14.30	Conditional gene targeting Barry Rosen (Hinxton, UK)
14.30 - 15.00	Conditional gene trapping Frank Schnütgen (Frankfurt, Germany)
15.00 - 15.30	Recombinase mediated cassette exchange in ES cells Ralf Kühn (Neuherberg, Germany)
15.30 - 16.00	Inducible systems for conditional mutagenesis Rolf Sprengel (Heidelberg, Germany) MPEG-4 Video File
16.00 - 16.30	Coffee break
16.30 - 17.00	Derivation of mice from mutant ES cells William C. Skarnes (Hinxton, UK)
17.00 - 17.30	Conditional RNAi gene knock downs in mice Jost Seibler (Köln, Germany)
17.30	Wine and cheese

Outcome

The course had 65 participants and was a great success. The talks from the course are added above. Click on a talk title above to open the pdf version of the presentation.

 

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